Queensland Brain Institute

The Queensland Brain Institute (QBI) Advanced Microscopy Facility is one of the largest and most diverse light microscopy facilities in the Southern Hemisphere. Built around 24 high-end instruments, it offers researchers access to state-of-the-art imaging technology, leading visualisation software and experienced staff for training, support and experiment advice. The facility’s capabilities allow 3D imaging of whole brains through to sub-cellular structures within neurons with techniques such as:

  • single-photon/two-photon scanned light-sheet microscopy
  • structured illumination microscopy (SIM)
  • single-molecule localisation microscopy (PALM/STORM/sptPALM)
  • single-molecule reconstruction and tracking
  • spinning disk confocal microscopy
  • laser-scanning confocal and two-photon microscopy
  • FLIM
  • FRAP
  • calcium imaging
  • stereology
  • automatic slide scanning.

Many of the instruments have been designed to allow imaging of live neurons and organisms to allow the study of dynamic processes related to neuroscience research, such as axon regeneration and neuronal survival. Researchers are supported by cloud-based deconvolution and image segmentation and analysis using Huygens Professional, Ilastik, CellProfiler, Imaris and Amira hosted on a GPU cluster and remote access data storage and management.

Website: http://web.qbi.uq.edu.au/microscopy/
To apply, click here

The Shadowing Offer

Main focus

Aspects related to user access

Core facility management

Quality Management

Software tools

Image data storage and analysis

Hands-on Custom-built two-photon scanned light-sheet microscope, LSM 710 confocal/two-photon, Elyra SIM/PALM/STORM, Roper iLas2 sptPALM, Diskovery spinning disk confocal, Yokogawa spinning disk confocal, Metafer automatic slide scanners and various wide-field instruments

Other Activities

Weekly neuroscience seminars

Other seminars, workshops, symposia at the University of Queensland

Preferred position of applicant

Facility manager

Imaging specialist

Duration of stay

2 weeks