Electron Microscopy Facility at the Instituto Gulbenkian de Ciência

Oeiras, Portugal

The Electron Microscopy Facility at Instituto Gulbenkian de Ciência (Oeiras, Portugal) aims to provide centralized, high-quality electron microscopy techniques and resources for all researchers at the Instituto Gulbenkian de Ciência. Its objective is also to collaborate with the local, national and international the scientific community to develop and improve techniques and overall knowledge concerning electron microscopy.
Below is a list of the equipment available for use in the Instituto Gulbenkian de Ciência Electron Microscopy Facility.

Below you can find a short overview of the facility equipment found at the Electron Microscopy Facility at the Instituto Gulbenkian de Ciência.

Sample Preparation Equipment

  • Processing Fumehood
  • Q150T ES Carbon Coater
  • EM Reichert Knifemaker
  • Stereomicroscopes
  • PELCO Biowave Sample Processing System with SteadyTemp Pro
  • Wholwend Compact 2 High Pressure Freezer
  • Leica AFS2 with Robot

Ultramicrotomes

  • EM Reichert Ultramicrotome
  • Leica UC7 / FC7 Ultramicrotome

Electron Microscopes

  • H-7650 TEM from Hitachi
  • Tecnai G2 Spirit BioTWIN TEM from FEI
  • Light Microscope for CLEM Experiments
  • Olympus IX81 Fluorescence Microscope

List of techniques that are offered by the facility

  • 2D Transmisson electron microscopy
  • Correlative Light and Electron Microscopy (CLEM)
  • Cryo and Room Temperature Sample Processing
  • Negative Staining
  • Tokuyasu
  • Immunolabeling
  • Electron Tomography

Facility webpage can be found here.

The Shadowing Offer

Main focus

Aspects related to user access

Quality management

Core facility management

Other Activities

PI seminars

Weekly invited external speaker seminars

Duration of stay

1-2 weeks depending on the activity (to be defined in advance)