This project has been made possible in part by a grant from the Chan Zuckerberg Initiative DAF, an advised fund of Silicon Valley Community Foundation.
This webinar series will introduce Scanning Electron Microscopy (SEM) and X-ray Imaging including fundamentals of SEM, X-ray imaging basics, sample preparation in life sciences, and applications of SEM in three-dimensions.
Imaging core facility staff including personnel of light, electron, or biomedical imaging facilities are invited to attend the webinars. Principal investigators, post-doctoral researchers, and graduate/PhD and Master's students who utilize imaging and microscopy technologies are also invited to attend.
Eligibility: everyone is welcome to register and attend the series
To register, please complete the form at the following link:
https://www.surveymonkey.com/r/gbi-zeiss-sem-2022
Dr. Aubrey Funke, Product Marketing Manager Life Science Electron Microscopy, ZEISS North America
Scanning Electron Microscopy (SEM) has been long established as a powerful and valuable technique for life science research for samples as varied as insects, tissues, organoids, cells and plants. This training will provide an introduction into how SEM technology works, the kind of applications that can be addressed using SEM approaches and the challenges that can limit the quality of the resulting images.
Dr. Rosy Manser, Solution Manager Life Science X-Ray Microscopy, ZEISS and Dr. Liz Duke, Team Leader for Biological X-ray Imaging EMBL Hamburg, Germany (Dr. Duke's team page)
The use of X-rays for imaging life science specimens is rapidly growing in popularity due to the high-resolution visualization of internal 3D structures that can be achieved without cutting the sample alongside the increasing availability of staining and mounting protocols. This training will provide a basic introduction to 3D X-Ray imaging and how it can be used in life science as well as an overview of the different opportunities possible both in the lab and at the synchrotron.
Dr. Kirk Czymmek, Director of the Advanced Bioimaging Laboratory, Donald Danforth Plant Science Center, USA (Dr. Czymmek facility page)
Preparing samples for electron microscopy can be something of a dark art! Generating the right level of fixation, a smooth embedding and perfect staining takes expertise and practice and many written protocols are not self-explanatory if you don’t have previous experience. This training will walk you through the basics of how to prepare your sample for SEM and discuss some of the common challenges that can limit the quality of the resulting specimen
Dr. Philipp Bastians, Sales Manager Life Science Electron & X-Ray Microscopy, ZEISS North America
The value of SEM in providing high resolution structural information in a wide range of life science samples has long been appreciated. Developments in technology and sample management capabilities also now enable the scaling up of these insights into far larger 2D areas as well as 3D volumes. The scaling up of SEM acquisitions provides exciting opportunities for new insights and experiments. This training will introduce the array of approaches that can expand SEM acquisition to larger areas and 3D volumes and discuss the challenges and opportunities that each approach provides.
