Instrument Operation

Virtual Beginner

Instrument Operation modules are dedicated to hands-on operation of microscopy systems.

Wide-field microscopy training (23 topics)
User beginner 36 mins 44 secs
Last updated 6 months ago

This module invites you to learn the operation of a wide-field epi-fluorescence microscope. This general training applies to a wide range of wide-field microscopes.

  • Training Series Overview
  • Overview of transmitted light microscopy techniques: Brightfield, Phase contrast, and Differential interference contrast
  • Overview of fluorescence imaging
  • Comparing upright and inverted microscopes
  • Microscope components and light paths
Starting the system
  • Starting up the microscope
  • Function of the Automatic Microscope Controller (AMC) unit
  • Control of lamps by the AMC unit
  • Timing of lamp use
Fluorescence lamp operation
  • The different lamps attached to microscopes around the facility
  • How to change the intensity of the light
  • How to illuminate your sample
  • How to troubleshoot
Sample mount and focus
  • Mounting the sample on the microscope
  • Adjusting the lamp for your sample
  • Focusing on your sample
  • Focus lock
  • Tips on finding your sample
  • Dry objectives
  • Numerical aperture (NA)
  • Immersion objectives and immersion oils
  • Cleaning objectives before and after imaging
  • Cleaning your slide
Brightfield imaging
  • Adjusting the aperture stop based on your objective for contrast
  • Setting the microscope component for imaging
  • Enhancing contrast of the image
Phase contrast imaging
  • Checking you have a phase contrast objective
  • Choosing the correct phase ring on the condenser
  • Adjusting the microscope components for high contrast
Differential Interference Contrast (DIC) imaging
  • Theory of DIC imaging
  • Microscope components required for DIC imaging
  • Examples of DIC images
  • DIC components that can effect fluorescence imaging
Fluorescence imaging
  • Examples of fluorescence probes
  • Microscope components needed for fluorescence imaging
  • Epifluorescence light source optimization
  • Troubleshooting tips
Image acquisition NIS-F elements
  • Opening the software
  • Camera operation
  • Changing viewing and acquistion resolution
  • Optimizing the contrast in your image
  • Adding and adjusting the scale bar
  • Autoexposure function
  • Focusing tips
  • Creating an RGB image from 3 separate images
  • Saving your images
Source material

Institute for Molecular Bioscience Microscopy

Zeiss Axio Imager 2 with Apotome training (24 topics)
User beginner 53 mins 52 secs
Last updated 6 months ago
Zeiss laser scanning confocal microscopy training (13 topics)
User beginner 32 mins 47 secs
Last updated 6 months ago