Euro-BioImaging EMBL Node

Euro-BioImaging EMBL Node features both ligth and electron microscopy state-of-the-art technologies and services. It consists of Advanced Light Microscopy Facility (ALMF) and Electron Microscopy Core Facility (EMCF).

Advanced Light Microscopy Facility offers a collection of state-of-the-art light microscopy equipment and image processing tools, supporting in-house scientists and visitors in using light microscopy methods for their research. The ALMF also regularly organises in-house and international courses to teach basic and advanced light microscopy methods and image analysis. It currently manages more than 20 advanced light microscopy systems and 10 high content microscopes from leading industrial companies. Several workstations for image analysis are also provided.

For more information on the ALMF see this page.

Electron Microscopy Core Facility is home to 4 TEM’s and 2 SEM’s together with advanced equipment for sample preparation. We cover a large spectrum of EM techniques with a major focus on sample preparation, ultrastructural analysis in 2D and 3D, correlative light and electron microscopy (CLEM) and data processing. The EMCF hosts a number of international and internal courses per year focusing on diverse EM techniques, with the content ranging from sample preparation to image analysis. Likewise, we offer several training opportunities for in-house scientists and external users of the facility.

For more information on the EMCF, see this page.

In the framework of the Global BioImaging job shadowing program, the following techniques are offered as hands-on at Advanced Light Microscopy Facility:

• Deconvolution widefield microscopy
• Laser scanning confocal microscopy (LSCM)
• Spinning disc confocal microscopy
• Multiphoton microscopy systems
• Total internal reflection fluorescence microscopy (TIRF)
• Fluorescence cross-correlation spectroscopy (FCCS)
• Fluorescence correlation spectroscopy (FCS)
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence resonance energy transfer (FRET)
• Correlative light electron microscopy (CLEM)
• High Throughput Microscopy
• Ground state depletion microscopy (GSD)
• Reversible saturable optical fluorescence transitions (RESOLFT)
• Stimulated emission depletion microscopy (STED)
• Stochastic optical reconstruction microscopy (STORM)
• Selective plane illumination microscopy (SPIM)
• Digital scanned laser light-sheet fluorescence microscopy (DSLM).

The following techniques are offered as hands-on at Electron Microscopy Core Facility:

• 3D EM imaging (TEM tomography, serial-section TEM, FIB-SEM).
• Focussed Ion Beam milling combined with Scanning Electron Microscopy (FIB-SEM)
• Correlative Light and Electron Microscopy (on section CLEM, cryo-CLEM, 3D CLEM)
• Immunoelectron microscopy

Websites:
https://www.embl.de/services/core_facilities/almf/
https://www.embl.de/services/core_facilities/em/
To apply, click here