Advanced Light Microscopy Facility of the European Molecular Biology Laboratory

The Advanced Light Microscopy Facility (ALMF) offers a collection of state-of-the-art light microscopy equipment and image processing tools, supporting in-house scientists and visitors in using light microscopy methods for their research. The ALMF also regularly organises in-house and international courses to teach basic and advanced light microscopy methods. It currently manages 20 advanced microscope systems and 10 high-content microscopes from leading industrial companies. Several workstations for image analysis are provided.

In the framework of the Global BioImaging job sjhadowing program, the following techniques are offered for hands-on:

  • Deconvolution widefield microscopy
  • Laser scanning confocal microscopy (LSCM)
  • Electron microscopy
  • Multiphoton microscopy systems
  • Spinning disc confocal systems
  • Total internal reflection fluorescence microscopy (TIRF)
  • Fluorescence cross-correlation spectroscopy (FCCS)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence recovery after photobleaching (FRAP)
  • Fluorescence resonance energy transfer (FRET)
  • Correlative light electron microscopy (CLEM)
  • High Throughput Microscopy
  • Ground state depletion microscopy (GSD)
  • Reversible saturable optical fluorescence transitions (RESOLFT)
  • Stimulated emission depletion microscopy (STED)
  • Stochastic optical reconstruction microscopy (STORM)
  • Selective plane illumination microscopy (SPIM)
  • Digital scanned laser light-sheet fluorescence microscopy (DSLM).

To apply, click here

The Shadowing Offer

Main focus

Aspects related to user access

Core facility management

Software tools

Image data storage and analysis


Other Activities

Open seminars at EMBL

Workshops, courses, conferences (via subscription, subject to selection)

Duration of stay

Varies, to be defined in advance on a case-by-case basis