Advanced Light Microscopy Facility of the European Molecular Biology Laboratory

The Advanced Light Microscopy Facility (ALMF) offers a collection of state-of-the-art light microscopy equipment and image processing tools, supporting in-house scientists and visitors in using light microscopy methods for their research. The ALMF also regularly organises in-house and international courses to teach basic and advanced light microscopy methods. It currently manages 20 advanced microscope systems and 10 high-content microscopes from leading industrial companies. Several workstations for image analysis are provided.

In the framework of the Global BioImaging job sjhadowing program, the following techniques are offered for hands-on:

  • Deconvolution widefield microscopy
  • Laser scanning confocal microscopy (LSCM)
  • Electron microscopy
  • Multiphoton microscopy systems
  • Spinning disc confocal systems
  • Total internal reflection fluorescence microscopy (TIRF)
  • Fluorescence cross-correlation spectroscopy (FCCS)
  • Fluorescence correlation spectroscopy (FCS)
  • Fluorescence recovery after photobleaching (FRAP)
  • Fluorescence resonance energy transfer (FRET)
  • Correlative light electron microscopy (CLEM)
  • High Throughput Microscopy
  • Ground state depletion microscopy (GSD)
  • Reversible saturable optical fluorescence transitions (RESOLFT)
  • Stimulated emission depletion microscopy (STED)
  • Stochastic optical reconstruction microscopy (STORM)
  • Selective plane illumination microscopy (SPIM)
  • Digital scanned laser light-sheet fluorescence microscopy (DSLM).

Website: https://www.embl.de/services/core_facilities/almf/index.html
To apply, click here

The Shadowing Offer

Main focus

Aspects related to user access

Core facility management

Software tools

Image data storage and analysis

Hands-on

Other Activities

Open seminars at EMBL

Workshops, courses, conferences (via subscription, subject to selection)

Duration of stay

Varies, to be defined in advance on a case-by-case basis