The Fluorescence Microscopy Unit is one of the units of the Central Analytical Facilities at Stellenbosch University, focusing on fluorescence microscopy and flow cytometry. Fluorescence-based instruments are used to assess the various properties of cells, particles or molecules of interest in a wide variety of samples.
The unit started with an epifluorescent Olympus IX81 microscope in 2006, and expanded infrastructure in 2012 with a Zeiss LSM780 with Elyra SR SIM platform. In 2015, this microscope was upgraded to add the PALM/dSTORM module and the Shuttle and Find feature.
Several protocols for Correlative Light and Electron Microscopy are also on place, in collaboration with the Electron Microscopy Unit.
The infrastructure is still under implementation and is currently expanding the unit to Tygerberg Medical Campus with a Zeiss AxioObserver fluorescence microscope. An Imaging Flow Cytometer will also be available by the end of 2019.
Further details can be found on the Unit's web page.
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Aspects related to user access
Core facility management
Hands-on Zeiss LSM 780 Elyra PS1 confocal/super-resolution microscope; Olympus IX81 inverted microscope; Zeiss AxioObserver
CLEM, super-resolution microscopy
Periodical confocal microscopy workshops
Up to 2 weeks