Harvard Center for Biological Imaging

The Harvard Center for Biological Imaging (HCBI) was opened May 17, 2010 and is one of the most innovative biological imaging centers in the world.
In order to ensure investigators always have access to the latest and best technologies, the HCBI was designed to be an “Evergreen” microscopy facility. Imaging systems at the HCBI are leased rather than purchased and replaced every 2-3 years. This ensures that the facility remains relevant and is never in possession of outdated equipment.
Currently, the HCBI contains 18 microscopes and high-end processing stations including:

  • Confocal (Zeiss LSM 700, LSM 880 inverted with FLIM, LSM 880 inverted with Airyscan and live cell incubation, LSM 880 upright),
  • super-resolution (Zeiss ELYRA7 with Lattice 3D Structured Illumination and 3D Single Molecular Localization),
  • multi-photon (Zeiss LSM 980 NLO),
  • Zeiss lightsheet.Z1,
  • live cell & high-content imaging (Zeiss Cell Discoverer 7),
  • slide scanning (Zeiss AxioScan.Z1 x2),
  • PALM laser dissection microscope and Zeiss AxioZoom.

Both lighsheet and upright confocal/multiphoton systems can be used with live water immersion or cleared tissue immersion objectives.

The facility also features wet lab space, eukaryotic and prokaryotic incubation, and access to nearby animal care facilities for a wide range of species.

Website: https://hcbi.fas.harvard.edu/
To apply, click here

The Shadowing Offer

Main focus

Aspects related to user access

Quality management

Core facility management

Software tools

Image data storage and analysis

Hands-on: see instruments at https://hcbi.fas.harvard.edu/equipment

Other Activities

Biweekly Lunch and Learn seminars during the Fall semester and Summer

Two day intensive microscopy course during January winter session

Hands on tissue clearing workshop

Data analysis workshops at various times of the year

Departmental weekly seminars, including Cell Dynamics (Systems Biology), Molecular and Cellular Biology, Center for Brain Science (Neuroscience), and Chemistry and Chemical Biology that are open to guests

Preferred position of applicant

Facility manager

Imaging specialist

Duration of stay

1-2 weeks